Dempsey suffered a right calf strain in training Wednesday morning in Glasgow, while Johnson has a persistent right ankle injury that will require further evaluation. No replacements will be added to the squad which now stands at 21 players.
The U.S. first faces Scotland on November 15 at Hampden Park in Glasgow, followed four days later by the friendly with Austria at Ernst-Happel-Stadion in Vienna.
The United States is concluding a tremendous year, highlighted by reaching the World Cup final competition for the seventh-consecutive time and a first place finish in qualifying in the CONCACAF region.
Below is the current U.S. Roster by position:
GOALKEEPERS (3): Bill Hamid (D.C. United), Tim Howard (Everton), Sean Johnson (Chicago Fire)
DEFENDERS (7): DaMarcus Beasley (Puebla), John Brooks (Hertha Berlin), Geoff Cameron (Stoke City), Brad Evans (Seattle Sounders FC), Omar Gonzalez (LA Galaxy), Eric Lichaj (Nottingham Forest), Michael Orozco (Puebla)
MIDFIELDERS (6): Alejandro Bedoya (FC Nantes), Michael Bradley (Roma), Mix Diskerud (Rosenborg), Jermaine Jones (Schalke), Sacha Kljestan (Anderlecht), Brek Shea (Stoke City)
FORWARDS (5): Jozy Altidore (Sunderland), Terrence Boyd (Rapid Vienna), Aron Johannsson (AZ Alkmaar), Eddie Johnson (Seattle Sounders FC), Chris Wondolowski (San Jose Earthquakes)
By Staff of The Daily Sports Herald and news services